SOD2 Antikörper

Produktnummer ABIN361656

Der Kaninchen Polyklonal Anti-SOD2-Antikörper wurde für WB, IHC, ELISA, IP, IF und ICC validiert. Er ist geeignet, SOD2 in Proben von Ratte zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für SOD2 Antikörper (ABIN361656)

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SOD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-SOD2 Antikörper

Spezifität: Detects ~25 kDa.

Kreuzreaktivität: Huhn, Rind (Kuh), Hund, Drosophila melanogaster, Meerschweinchen, Hamster, Human, Invertebrat, Affe, Maus, Schwein, Kaninchen, Ratte, Sea Squirt, Schaf, Eichhörnchen, Xenopus laevis

Aufreinigung: Protein A Purified

Immunogen: Recombinant Rat Mn SOD Protein

Anwendungsinformationen

Applikationshinweise:

• WB (1:5000)
• IHC (1:100)
• ICC/IF (1:120)
• optimal dilutions for assays should be determined by the user.

Kommentare:

0.5 μg/ml of ABIN361655 was sufficient for detection of Mn SOD in 20 μg of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-rabbit IgG:AP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Referenzen für anti-SOD2 Antikörper (ABIN361656)

Allan, Storey: "Expression of NF-?B and downstream antioxidant genes in skeletal muscle of hibernating ground squirrels, Spermophilus tridecemlineatus." in: Cell biochemistry and function, Vol. 30, Issue 2, pp. 166-74, (2012) (PubMed).

Ishii, Kunihiro, Tanaka, Hatano, Nishikata: "Cytosolic subunits of ATP synthase are localized to the cortical endoplasmic reticulum-rich domain of the ascidian egg myoplasm." in: Development, growth & differentiation, Vol. 54, Issue 8, pp. 753-66, (2012) (PubMed).

Wu, Hsiao, Chien, Lai: "Ischemic conditioning by short periods of reperfusion attenuates renal ischemia/reperfusion induced apoptosis and autophagy in the rat." in: Journal of biomedical science, Vol. 16, pp. 19, (2009) (PubMed).

Details zu SOD2

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Andere Bezeichnung: SOD2

Hintergrund: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).

Gen-ID: 24787

NCBI Accession: NP_058747

UniProt: P07895

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Negative Regulation of intrinsic apoptotic Signaling