SOD1 Antikörper

Produktnummer ABIN361652

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SOD1 in WB, IHC, ELISA, IP, ICC und IF. Er zeigt eine Reaktivität gegenüber Ratte und wurde in 4+ Publikationen erwähnt.

Kurzübersicht für SOD1 Antikörper (ABIN361652)

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SOD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-SOD1 Antikörper

Spezifität: Detects ~23 kDa (human) and ~19 kDa (other species).

Kreuzreaktivität: Rind (Kuh), Fusarium, Human, Maus, Ratte

Aufreinigung: Protein A Purified

Immunogen: Synthetic Rat Cu/Zn SOD Peptide

Anwendungsinformationen

Applikationshinweise:

• WB (1:2000)
• ICC/IF (1:120)
• optimal dilutions for assays should be determined by the user.

Kommentare:

0.5 μg/ml of ABIN361651 was sufficient for detection of Cu/Zn SOD in 20 μg of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-rabbit IgG:AP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.0, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Referenzen für anti-SOD1 Antikörper (ABIN361652)

Furukawa, Sakuda: "Inhibition of Aflatoxin Production by Paraquat and External Superoxide Dismutase in Aspergillus flavus." in: Toxins, Vol. 11, Issue 2, (2019) (PubMed).

Hung, Li, Lee, Chang, Chien, Lee: "Green tea extract supplementation ameliorates CCl4-induced hepatic oxidative stress, fibrosis, and acute-phase protein expression in rat." in: Journal of the Formosan Medical Association = Taiwan yi zhi, Vol. 111, Issue 10, pp. 550-9, (2012) (PubMed).

Wang, Kumagai-Braesch, Herrington, Larsson, Permert: "Increased lipid metabolism and cell turnover of MiaPaCa2 cells induced by high-fat diet in an orthotopic system." in: Metabolism: clinical and experimental, Vol. 58, Issue 8, pp. 1131-6, (2009) (PubMed).

Huang, Lee, Kuo, Yang, Chien: "Attenuation of long-term Rhodiola rosea supplementation on exhaustive swimming-evoked oxidative stress in the rat." in: The Chinese journal of physiology, Vol. 52, Issue 5, pp. 316-24, (2009) (PubMed).

Details zu SOD1

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Andere Bezeichnung: SOD1

Hintergrund: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).

Gen-ID: 24786

NCBI Accession: NP_058746

UniProt: P07632

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis