Progesterone Receptor Antikörper (pSer190)

Produktnummer ABIN361517

Der Maus Monoklonal Anti-Progesterone Receptor-Antikörper wurde für WB und IHC validiert. Er ist geeignet, Progesterone Receptor in Proben von Human zu detektieren.

Kurzübersicht für Progesterone Receptor Antikörper (pSer190) (ABIN361517)

Target: Progesterone Receptor (PGR)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Progesterone Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: 1154

Produktdetails anti-Progesterone Receptor Antikörper

Bindungsspezifität: pSer190

Spezifität: Specific for the ~90k PR-A isoform and the ~120k PR-B isoform phosphorylated at Ser190. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.

Kreuzreaktivität: Human

Aufreinigung: Protein G purified culture supernatant

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser190 conjugated to KLH

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:1000 IHC (frozen sections, unpublished observations): 1:1000 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Details zu Progesterone Receptor

Target: Progesterone Receptor (PGR)

Andere Bezeichnung: PGR

Hintergrund: There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer (Edwards, 2005). The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (PR-A and PR-B forms). In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones (Attia et al., 2000, Lin et al., 2003). Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor (Clemm et al., 2000) . Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser190 and Ser294 with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor. Anti-Phospho Ser190 Progesterone Receptor Western blot of whole cell T47D lysate prepared from cells that had been inubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of the ~90k PR-A isoform and the ~120 PR-B isoform of the progesterone receptor phosphorylated at Ser190. The immunolabeling is specifically blocked by the phosphopeptide used as the antigen (not shown).

Molekulargewicht: 90/120 kDa

Gen-ID: 5241

UniProt: P06401

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Smooth Muscle Cell Migration