IFNAR1 Antikörper (pSer535, pSer539)

Produktnummer ABIN361505

Der Kaninchen Polyklonal Anti-IFNAR1-Antikörper wurde für WB und IHC validiert. Er ist geeignet, IFNAR1 in Proben von Human zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für IFNAR1 Antikörper (pSer535, pSer539) (ABIN361505)

Target: IFNAR1 (Interferon alpha/beta Receptor 1 (IFNAR1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IFNAR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-IFNAR1 Antikörper

Bindungsspezifität: pSer535, pSer539

Spezifität: Specific for IFNAR1 protein phosphorylated at Ser535,539.

Kreuzreaktivität: Human

Homologie: bovine, canine, mouse, non-human primate, rat, sheep

Aufreinigung: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser535/539 conjugated to KLH

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:1000 IHC: 1:1000 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Referenzen für anti-IFNAR1 Antikörper (ABIN361505)

Bhattacharya, HuangFu, Liu, Veeranki, Baker, Koumenis, Diehl, Fuchs: "Inducible priming phosphorylation promotes ligand-independent degradation of the IFNAR1 chain of type I interferon receptor." in: The Journal of biological chemistry, Vol. 285, Issue 4, pp. 2318-25, (2010) (PubMed).

Kumar, Krolewski, Fuchs: "Phosphorylation and specific ubiquitin acceptor sites are required for ubiquitination and degradation of the IFNAR1 subunit of type I interferon receptor." in: The Journal of biological chemistry, Vol. 279, Issue 45, pp. 46614-20, (2004) (PubMed).

Details zu IFNAR1

Target: IFNAR1 (Interferon alpha/beta Receptor 1 (IFNAR1))

Andere Bezeichnung: IFNAR1

Hintergrund: Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (Biron, 2001, Kirkwood, 2002). These cytokines produce their effects by binding to the Type 1 Interferon-a Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine 535 and 539 in the IFNAR1 (Kumar et al., 2003). Anti-Phospho Ser535,539 IFNAR1 Western blot of immunoprecipitates from HEK 293 cells transfected with 1. Mock, 2. IFNAR1 WT, and 3. IFNAR1 S535A and S539A mutants showing specific immunolabeling of the ~110k to ~130k IFNAR1 WT. The immunolabeling is absent in IFNAR1 Ser535 and Ser539 mutants (Control). The immunolabeling is blocked by the phosphopeptide (Phos) used as the antigen but not by the corresponding dephosphopeptide (Dephos).

Molekulargewicht: 110-130 kDa

Gen-ID: 3654

UniProt: P17181

Pathways: JAK-STAT Signalweg, Hepatitis C