SYN1 Antikörper (pSer549)

Produktnummer ABIN361492

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SYN1 in WB und IHC. Er zeigt eine Reaktivität gegenüber Ratte und wurde in 1 Publikation erwähnt.

Kurzübersicht für SYN1 Antikörper (pSer549) (ABIN361492)

Target: SYN1 (Synapsin I (SYN1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SYN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-SYN1 Antikörper

Bindungsspezifität: pSer549

Spezifität: Specific for ~78k synapsin I doublet phosphorylated at Ser549. Immunolabeling of the synapsin I band is blocked by (-phosphatase treatment.

Kreuzreaktivität: Ratte (Rattus)

Homologie: bovine, canine, human, mouse, non-human primate

Aufreinigung: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser549 conjugated to KLH

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:1000 IHC: 1:500 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Referenzen für anti-SYN1 Antikörper (ABIN361492)

Dagyte, Luiten, De Jager, Gabriel, Mocaër, Den Boer, Van der Zee: "Chronic stress and antidepressant agomelatine induce region-specific changes in synapsin I expression in the rat brain." in: Journal of neuroscience research, Vol. 89, Issue 10, pp. 1646-57, (2011) (PubMed).

Details zu SYN1

Target: SYN1 (Synapsin I (SYN1))

Andere Bezeichnung: SYN1

Hintergrund: Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002, Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001, Kao et al., 2002). Ser 549 along with Ser 62 and Ser 67 are the sites of synapsin I that are phosphorylated by MAP kinase (Jovanovic et al., 1996). Phosphorylation and subsequent dephosphorylation of this site is thought to play a key role in synaptic vesicle trafficking. Anti-Phospho-Ser549 Synapsin Western blot of rat cortex lysate showing specific immunolabeling of the ~78k synapsin I phosphorylated at Ser549 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the phospho Ser549 synapsin I antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.

Molekulargewicht: '78 kDa

Gen-ID: 281510

UniProt: P17599