EPH Receptor B2 Antikörper (pTyr317)

Produktnummer ABIN361467

Dieses Anti-EPH Receptor B2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von EPH Receptor B2 in WB. Geeignet für Huhn.

Kurzübersicht für EPH Receptor B2 Antikörper (pTyr317) (ABIN361467)

Target: EPH Receptor B2 (EPHB2)

Reaktivität: Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EPH Receptor B2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-EPH Receptor B2 Antikörper

Bindungsspezifität: pTyr317

Spezifität: Specific for the ~46k EphrinB protein phosphorylated at Tyr317. The immunolabeling of the EphrinB band is blocked by treatment with (-phosphatase.

Kreuzreaktivität: Maus, Ratte (Rattus)

Homologie: bovine, chicken, human, mouse, Xenopus, zebra fish

Aufreinigung: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr317 conjugated to KLH

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Details zu EPH Receptor B2

Target: EPH Receptor B2 (EPHB2)

Andere Bezeichnung: EPHB2

Hintergrund: EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vanderhaeghen, 1998, Dufour et al., 2003, Oike et al., 2002). EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr298) plays a key role in EphrinB signaling (Kalo, et al., 2001). Anti-Phospho-Tyr317 EphrinB Western blot of rat testes lysate showing specific immunolabeling of the ~46k EphrinB phosphorylated at Tyr317 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the Anti-Tyr317 EphrinB. The immunolabeling of the EphrinB is completely eliminated by treatment with (-Ptase.

Molekulargewicht: '46 kDa

Gen-ID: 396513

UniProt: P28693

Pathways: RTK Signalweg, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteine