GABRA3 Antikörper (N-Term)

Produktnummer ABIN361437

Dieses Anti-GABRA3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von GABRA3 in WB und IHC. Geeignet für Ratte.

Kurzübersicht für GABRA3 Antikörper (N-Term) (ABIN361437)

Target: GABRA3 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GABRA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-GABRA3 Antikörper

Bindungsspezifität: N-Term

Spezifität: Specific for the ~51k a3-subunit of the GABAA receptor in Western blots. Labeling is absent in a3-subunit knockout animals.

Kreuzreaktivität: Maus, Ratte (Rattus)

Homologie: bovine, canine, human, non-human primates, Zebrafish

Aufreinigung: Antigen Affinity Purified

Immunogen: Synthetic peptide from the N-terminal region of the alpha 3 subunit

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Details zu GABRA3

Target: GABRA3 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3))

Andere Bezeichnung: GABRA3

Hintergrund: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, fourbetas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990, Whiting et al., 1999, Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and gamma-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a -subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different a-subunits of the receptor (McKernan et al., 2000, Mehta and Ticku, 1998, Ogris et al., 2004, P ltl et al., 2003). Anti-GABAA-Receptor, 3 -Subunit Western blot of mouse brain lysates from wild type (Control) and a3-knockout (aK/O) animals showing specific immunolabeling of the ~51k a3-subunit of the GABAA-R. The labeling was absent from a lysate prepared from a3-knockout animals.

Molekulargewicht: '51 kDa

Gen-ID: 24947

UniProt: P20236