Metabotropic Glutamate Receptor 1 Antikörper (C-Term)

Produktnummer ABIN361416

Dieses Anti-Metabotropic Glutamate Receptor 1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Metabotropic Glutamate Receptor 1 in WB und IHC. Geeignet für Ratte.

Kurzübersicht für Metabotropic Glutamate Receptor 1 Antikörper (C-Term) (ABIN361416)

Target: Metabotropic Glutamate Receptor 1 (GRM1) (Glutamate Receptor, Metabotropic 1 (GRM1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Metabotropic Glutamate Receptor 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Metabotropic Glutamate Receptor 1 Antikörper

Bindungsspezifität: C-Term

Spezifität: Specific for the ~125k monomer and the ~250k mGluR1a dimer. Immunolabeling blocked by preadsorption of antibody with the peptide used to generate the antibody.

Kreuzreaktivität: Maus, Ratte (Rattus)

Aufreinigung: Antigen Affinity Purified

Immunogen: Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:1000 IHC (frozen sections, unpublished observations): 1:500 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Details zu Metabotropic Glutamate Receptor 1

Target: Metabotropic Glutamate Receptor 1 (GRM1) (Glutamate Receptor, Metabotropic 1 (GRM1))

Andere Bezeichnung: GRM1

Hintergrund: The metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004, Francesconi et al., 2004, Wilson and Nicoll, 2001). Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast, activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase. The mGluR1 receptor may also be critically involved in limiting the deleterious effects of excitotoxicity (Blaabjerg et al., 2003). Anti-mGluR1a Western blot of 10 ug of HEK 293 cells expressing mGluR1a and mGluR5 showing the specific immunolabeling of the ~125k monomer and the ~250k dimer of mGluR1a. The mGluR1a antibody shows no reactivity toward mGluR5.

Molekulargewicht: 125/250 kDa

Gen-ID: 24414

UniProt: P23385