Fibrillarin Antikörper

Produktnummer ABIN361375

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Fibrillarin in WB und IF. Er zeigt eine Reaktivität gegenüber Saccharomyces cerevisiae und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für Fibrillarin Antikörper (ABIN361375)

Target: Fibrillarin (FBL)

Reaktivität: Saccharomyces cerevisiae

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Fibrillarin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 38F3

Produktdetails anti-Fibrillarin Antikörper

Spezifität: Specific for the ~34kDa Fibrillarin /Nop1p protein. It is expected that it will work on other mammal tissues.

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Aufreinigung: Protein G purified culture supernatant

Immunogen: Yeast nuclear preparations

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:1,000 IF: 1:500 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: total IgG fraction + 10 mM Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen für anti-Fibrillarin Antikörper (ABIN361375)

Vendramin, Verheyden, Ishikawa, Goedert, Nicolas, Saraf, Armaos, Delli Ponti, Izumikawa, Mestdagh, Lafontaine, Tartaglia, Takahashi, Marine, Leucci: "SAMMSON fosters cancer cell fitness by concertedly enhancing mitochondrial and cytosolic translation." in: Nature structural & molecular biology, Vol. 25, Issue 11, pp. 1035-1046, (2019) (PubMed).

Langhendries, Nicolas, Doumont, Goldman, Lafontaine: "The human box C/D snoRNAs U3 and U8 are required for pre-rRNA processing and tumorigenesis." in: Oncotarget, Vol. 7, Issue 37, pp. 59519-59534, (2018) (PubMed).

Details zu Fibrillarin

Target: Fibrillarin (FBL)

Andere Bezeichnung: Fibrillarin (Nop1p)

Hintergrund: Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli (1). The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin (2). Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable (3). This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).

Molekulargewicht: '34 kDa

Gen-ID: 851548

UniProt: P15646

Pathways: Ribonucleoside Biosynthetic Process