AtpH Antikörper

Produktnummer ABIN361271

Der Kaninchen Polyklonal Anti--Antikörper wurde für WB validiert. Er ist geeignet, in Proben von Arabidopsis thaliana und Chlamydomonas reinhardtii zu detektieren.

Kurzübersicht für AtpH Antikörper (ABIN361271)

Target: AtpH

Reaktivität: Arabidopsis thaliana, Chlamydomonas reinhardtii

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails

Kreuzreaktivität (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Homologie: dicots including Glycine max, Pisum sativum, Vitis vinifera, monocolts indlucing: Hordeum vulgare, Oryza sativa, Zea mays, trees: Populus alba, Pinus thunbergii, moss: Physcomitrella patens, green algae, Ostreococcus tauri

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 8 kDa (for Arabidopsis thaliana)

Aufreinigung: serum

Immunogen: KLH-conjugated peptides derived from AtpH subunit c of Arabidopsis thaliana P56760, AtCg00140 and Chlamydomonas reinhardtii Q37304

Anwendungsinformationen

Applikationshinweise: 1: 10 000 with standard ECL (WB)

Kommentare:

please note that increased incubation at 95°C (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e.g. 5 or 6 subunits) may occur

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 μL of sterile water

Handhabung: Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Lagerung: -20 °C

Informationen zur Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Antigendetails

Target: AtpH

Hintergrund: AGI Code: ATCG00140
F-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. Multiple copies of the c subunit build up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme.

Molekulargewicht: 8 kDa (for Arabidopsis thaliana)

UniProt: P56760, Q37304