AtpH Antikörper

Produktnummer ABIN249344

Der Kaninchen Polyklonal anti-AtpH Antikörper wird verwendet zum Nachweis von AtpH in Proben von Arabidopsis thaliana, Nicotiana benthamiana, Spinach und Thermosynechococcus elongatus. Er wurde validiert für WB.

Kurzübersicht für AtpH Antikörper (ABIN249344)

Target: AtpH

Reaktivität: Arabidopsis thaliana, Nicotiana benthamiana, Spinach, Thermosynechococcus elongatus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails

Verwendungszweck: Anti-AtpH | ATP synthase subunit c (chloroplastic)

Spezifität: The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthasesubcomplex, as well as the chloroplastic CF1 ATP synthase and most knownbacterial F-type ATP synthases.

Kreuzreaktivität (Details): Arabidopsis thaliana, Nicotiana benthamina, Spinacia oleracea, Thermosynechococcus elongatus
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Algae,Cannabis sativa,Cyclotella cryptica,Glycine max,Oryza sativa,Physcomitrium patens,Phaeodactylum tricornutum,Pisum sativum,Populus alba,Pinus thunbergii,Thalassiosira pseudonana,Zea mays,Vitis vinifera

Aufreinigung: Serum

Immunogen: Purified c subunit from Spinacia oleracea UniProt: P69447.

Anwendungsinformationen

Applikationshinweise: 1 : 1000-1 : 10 000 (WB)

Kommentare:

please note that increased incubation at 95°C (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e.g. 5 or 6 subunits) may occur

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 200 μL of sterile water

Buffer: Serum

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Antigendetails

Target: AtpH

Hintergrund:

Background: F-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. Multiple copies of the c subunit build up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme.

Additional information: This product can be sold containing proclin if requested.

Molekulargewicht: 8 kDa (for Arabidopsis thaliana)

Gen-ID: 2715579

UniProt: P69447