Podoplanin Antikörper

Produktnummer ABIN360956

Der Maus Monoklonal Anti-Podoplanin-Antikörper wurde für WB, IF, IHC (p) und IHC (fro) validiert. Er ist geeignet, Podoplanin in Proben von Ratte zu detektieren.

Kurzübersicht für Podoplanin Antikörper (ABIN360956)

Target: Podoplanin (PDPN)

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Podoplanin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: LF3(B7)D5B3

Produktdetails anti-Podoplanin Antikörper

Spezifität: This antibody recognizes Rat Podoplanin.

Aufreinigung: Protein G Chromatography

Immunogen: BALB/c mice using a membrane protein fraction of isolated Rat glomeruli

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 6.0 without preservatives and stabilizers

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at -20 °C. Following reconstitution store undiluted at 2-8 °C for two weeks or (in aliquots) at -20 °C for longer.

Details zu Podoplanin

Target: Podoplanin (PDPN)

Andere Bezeichnung: Podoplanin

Hintergrund: Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1-alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid precursor. It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207-228) and a short cytoplasmic tail (aa 229-238). The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47 % aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC-2). Their association is dependent on sialic acid on O-glycans of Podoplanin. Through its association with CLEC-2, Podoplanin induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.Synonyms: Aggrus, GP36, Glycoprotein 36, PA2.26 antigen, PDPN, PSEC0003, PSEC0025, T1-alpha

Gen-ID: 54320

NCBI Accession: NP_062231

UniProt: Q64294

Pathways: Dicarboxylic Acid Transport