ELISA: 1/1,000. Western Blot: 1/50 - 1/200. Immunohistochemistry: 1/50 - 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
0.25 mg/mL
Buffer
PBS with 0.09 % (W/V) sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Target
DGKA
(Diacylglycerol Kinase, alpha 80kDa (DGKA))
Andere Bezeichnung
DAG Kinase alpha
Hintergrund
Upon cell stimulation, the kinase DGKA converts the second messenger diacylglycerol into phophatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity. DGKA is stimulated by calcium and phosphatidylserine, and is phosphorylated by protein kinase C. Tissue expression is in lymphocytes and oligodengroglial cells. DGKA contains 2 zinc-dependent phorbol-ester and DAG binding domains, and 2 EF-hand calcium binding domains.Synonyms: DAGK, DAGK1, DGK-alpha, DGKA, Diacylglycerol kinase alpha, Diglyceride kinase alpha