EIF4E Antikörper (pSer209)

Produktnummer ABIN358406

Der Kaninchen Polyklonal Anti-EIF4E-Antikörper wurde für EIA validiert. Er ist geeignet, EIF4E in Proben von Human zu detektieren.

Kurzübersicht für EIF4E Antikörper (pSer209) (ABIN358406)

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EIF4E Antikörper ist unkonjugiert

Applikation: Enzyme Immunoassay (EIA)

Produktdetails anti-EIF4E Antikörper

Bindungsspezifität: pSer209

Spezifität: This antibody detectsEIF4E pSer209.

Aufreinigung: Affinity purification in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S209 of human EIF4E.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu EIF4E

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Andere Bezeichnung: EIF4E

Hintergrund: EIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E, insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs.Synonyms: EIF-4E, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein

Molekulargewicht: 25097 Da

Gen-ID: 1977, 9606

UniProt: P06730

Pathways: BCR Signaling