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p107 Antikörper (pSer975)

Dieses Anti-p107-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von p107 in WB, IHC (p) und EIA. Geeignet für Human.
Produktnummer ABIN358209

Kurzübersicht für p107 Antikörper (pSer975) (ABIN358209)

Target

Alle p107 (RBL1) Antikörper anzeigen
p107 (RBL1) (Retinoblastoma-Like 1 (p107) (RBL1))

Reaktivität

  • 73
  • 18
  • 9
  • 4
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Human

Wirt

  • 70
  • 3
Kaninchen

Klonalität

  • 71
  • 2
Polyklonal

Konjugat

  • 31
  • 6
  • 5
  • 5
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
Dieser p107 Antikörper ist unkonjugiert

Applikation

  • 60
  • 34
  • 27
  • 27
  • 10
  • 9
  • 7
  • 6
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
  • Bindungsspezifität

    • 18
    • 15
    • 8
    • 7
    • 7
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer975

    Spezifität

    This antibody detects Rb-like-1 pSer975.

    Aufreinigung

    Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

    Immunogen

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S975 of human Rb-like 1.

    Isotyp

    Ig Fraction
  • Applikationshinweise

    ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50approx. 1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    0.25 mg/mL

    Buffer

    PBS with 0.09 % (W/V) Sodium Azide as preservative.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C/-20 °C

    Informationen zur Lagerung

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Target

    p107 (RBL1) (Retinoblastoma-Like 1 (p107) (RBL1))

    Andere Bezeichnung

    RBL1

    Hintergrund

    RB-like 1 is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and RB-like 1 can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that RB-like 1 may also be a tumor suppressor.Synonyms: 107 kDa retinoblastoma-associated protein, Retinoblastoma-like protein 1, p107, pRb1

    Molekulargewicht

    120877 Da

    Gen-ID

    5933, 9606

    UniProt

    P28749

    Pathways

    Zellzyklus, Mitotic G1-G1/S Phases
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