p107 Antikörper (pSer975)
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- Target Alle p107 (RBL1) Antikörper anzeigen
- p107 (RBL1) (Retinoblastoma-Like 1 (p107) (RBL1))
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Bindungsspezifität
- pSer975
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser p107 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Spezifität
- This antibody detects Rb-like-1 pSer975.
- Aufreinigung
- Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Immunogen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S975 of human Rb-like 1.
- Isotyp
- Ig Fraction
- Top Product
- Discover our top product RBL1 Primärantikörper
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- Applikationshinweise
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ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.25 mg/mL
- Buffer
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Target
- p107 (RBL1) (Retinoblastoma-Like 1 (p107) (RBL1))
- Andere Bezeichnung
- RBL1 (RBL1 Produkte)
- Hintergrund
- RB-like 1 is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and RB-like 1 can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that RB-like 1 may also be a tumor suppressor.Synonyms: 107 kDa retinoblastoma-associated protein, Retinoblastoma-like protein 1, p107, pRb1
- Molekulargewicht
- 120877 Da
- Gen-ID
- 5933, 9606
- UniProt
- P28749
- Pathways
- Zellzyklus, Mitotic G1-G1/S Phases
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