RAD9A Antikörper (pSer272)

Produktnummer ABIN358200

Produktdetails anti-RAD9A Antikörper

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Bindungsspezifität: pSer272

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAD9A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Spezifität: This antibody detects Rad9 pSer272.

Aufreinigung: Protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S272 of human Rad9.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu RAD9A

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Andere Bezeichnung: RAD9A (RAD9A Produkte)

Synonyme: RAD9 antikoerper, Rad9 antikoerper, rad9a antikoerper, MGC89062 antikoerper, XRad9 antikoerper, rad9 antikoerper, RAD9 checkpoint clamp component A antikoerper, RAD9 checkpoint clamp component B antikoerper, RAD9 checkpoint clamp component A L homeolog antikoerper, RAD9A antikoerper, Rad9a antikoerper, rad9a antikoerper, rad9b antikoerper, rad9a.L antikoerper

Hintergrund: Rad9 is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade.Synonyms: Cell cycle checkpoint control protein RAD9A, DNA repair exonuclease rad9 homolog A, EC=3.1.11.2, RAD-9A

Molekulargewicht: 42547 Da

Gen-ID: 5883, 9606

UniProt: Q99638

Pathways: Positive Regulation of Response to DNA Damage Stimulus