PROX1 Antikörper (Center)

Produktnummer ABIN357418

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PROX1 in WB, IHC (p) und EIA. Er zeigt eine Reaktivität gegenüber Human und Maus und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für PROX1 Antikörper (Center) (ABIN357418)

Target: PROX1 (Prospero Homeobox 1 (PROX1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PROX1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-PROX1 Antikörper

Bindungsspezifität: Center

Spezifität: This antibody detects PROX1 at Center.

Aufreinigung: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. Then the antibody is purified by peptide affinity purification.

Immunogen: KLH conjugated synthetic peptide selected from the Center region of human PROX1

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: ELISA: 1/1,000. Western blot: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-PROX1 Antikörper (ABIN357418)

Park, Cho, Park, Kim, Park, Myung, Lee, Kweon, Joo: "Prospero homeobox 1 mediates the progression of gastric cancer by inducing tumor cell proliferation and lymphangiogenesis." in: Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association, Vol. 20, Issue 1, pp. 104-115, (2017) (PubMed).

Park, Myung, Park, Kim, Oak, Myung, Cho, Lee, Kweon, Kim, Joo: "Impact of prospero homeobox-1 on tumor cell behavior and prognosis in colorectal cancer." in: American journal of cancer research, Vol. 5, Issue 11, pp. 3286-300, (2016) (PubMed).

Details zu PROX1

Target: PROX1 (Prospero Homeobox 1 (PROX1))

Andere Bezeichnung: PROX-1

Hintergrund: The expression pattern of Prox1 suggests that it has a role in a variety of embryonic tissues, including lens. Prox mRNA is present in many different human tissues with lens demonstrating the highest level. Homozygous Prox1-null mice die at midgestation from multiple developmental defects, and a targeted effect on lens development has been reported. Prox1 inactivation caused abnormal cellular proliferation, downregulated expression of the cell cycle inhibitors Cdkn1b and Cdkn1c, misexpression of E-cadherin, and excessive apoptosis. Consequently, mutant lens cells failed to polarize and elongate properly, resulting in a hollow lens. Prox1 is expressed in a subpopulation of endothelial cells that by budding and sprouting give rise to the lymphatic system. Prox1 appears to be a specific and required regulator of the development of the lymphatic system. Prox1 also has been documented to be required for hepatocyte migration in the mouse. Loss of Prox1 results in a smaller liver with a reduced population of clustered hepatocytes. The homeodomain protein Prox1 regulates the egress of progenitor cells from the cell cycle in the embryonic mouse retina. Cells lacking Prox1 are less likely to stop dividing, and ectopic expression of Prox1 forces progenitor cells to exit the cell cycle. Prox1 acts as a key participant in progenitor-cell proliferation and cell-fate determination in the vertebrate retina.Synonyms: Homeobox prospero-like protein PROX1, PROX1, Prospero homeobox protein 1

Molekulargewicht: 83162 Da

Gen-ID: 5629, 9606

UniProt: Q92786

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation