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UBE2I Antikörper (N-Term)

Der Kaninchen Polyklonal Anti-UBE2I-Antikörper wurde für WB, IHC (p) und EIA validiert. Er ist geeignet, UBE2I in Proben von Human zu detektieren.
Produktnummer ABIN356594

Kurzübersicht für UBE2I Antikörper (N-Term) (ABIN356594)

Target

Alle UBE2I Antikörper anzeigen
UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Reaktivität

  • 79
  • 36
  • 36
  • 8
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 4
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
Human

Wirt

  • 65
  • 12
  • 2
Kaninchen

Klonalität

  • 54
  • 25
Polyklonal

Konjugat

  • 41
  • 7
  • 5
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
Dieser UBE2I Antikörper ist unkonjugiert

Applikation

  • 56
  • 32
  • 28
  • 11
  • 9
  • 8
  • 6
  • 6
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
  • Bindungsspezifität

    • 6
    • 6
    • 5
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    N-Term

    Spezifität

    This antibody is specific to UBE2I (N-term). Predicted to cross react with Mouse and DANRE (100 % Antigen Homology).

    Aufreinigung

    Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

    Immunogen

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human UBE2I.

    Isotyp

    Ig Fraction
  • Applikationshinweise

    ELISA: 1/1,000. Western blot: 1/100approx. 1/500. Immunohistochemistry: 1/50approx. 1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    0.25 mg/mL

    Buffer

    PBS containing 0.09 % (W/V) Sodium Azide as preservative.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C/-20 °C

    Informationen zur Lagerung

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Target

    UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

    Andere Bezeichnung

    UBC9 / UBE2I

    Hintergrund

    UBE2I (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumoylation, and may play a role in substrate recognition. UBE2I can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, IkBalpha, and PML without the requirement of an E3 ligase. UBE2I is essential for nuclear architecture and chromosome segregation.Synonyms: SUMO-conjugating enzyme UBC9, SUMO-protein ligase, UBCE9, Ubce2i, Ube2i, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I

    Gen-ID

    7329, 9606

    UniProt

    P63279

    Pathways

    Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway
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