HA-Tag Antikörper

Details zu Produkt Nr. ABIN356535, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
416
41
19
7
4
3
2
1
1
Wirt
Kaninchen
215
186
34
31
22
3
Klonalität
Polyklonal
Konjugat
Unkonjugiert
43
30
22
9
9
8
7
7
7
6
6
6
6
6
5
5
5
5
4
4
4
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Enzyme Immunoassay (EIA), Western Blotting (WB)
400
189
127
117
77
75
74
30
15
13
11
10
9
5
4
4
4
4
4
2
2
2
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen.
Sequenz YPYDVPDYA
Isotyp Ig Fraction
Reinigung Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Andere Bezeichnung HA Epitope Tag (YPYDVPDYA)
Substanzklasse Tag
Hintergrund Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYAYPYDVPDYA) is an established example. This polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells.
Applikations-hinweise ELISA: 1/5,000. Western Blot. Immunofluorescence. (See Ref.1, 4. )ChIP. (See Ref.3)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Bilder des Herstellers
 image for anti-HA-Tag antibody (ABIN356535) anti-HA-Tag antibody
Allgemeine Veröffentlichungen Scarselli, Donaldson: "Constitutive internalization of G protein-coupled receptors and G proteins via clathrin-independent endocytosis." in: The Journal of biological chemistry, Vol. 284, Issue 6, pp. 3577-85, 2009 (PubMed).

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