HA-Tag Antikörper
Kurzübersicht für HA-Tag Antikörper (ABIN356535)
Target
Wirt
Klonalität
Konjugat
Applikation
-
-
Sequenz
- YPYDVPDYA
-
Aufreinigung
- Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
-
Immunogen
- KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen.
-
Isotyp
- Ig Fraction
-
-
-
-
Applikationshinweise
-
ELISA: 1/5,000. Western Blot. Immunofluorescence. (See Ref.1, 4. )ChIP. (See Ref.3)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Format
- Liquid
-
Konzentration
- 0.25 mg/mL
-
Buffer
- PBS containing 0.09 % (W/V) Sodium Azide as preservative.
-
Konservierungsmittel
- Sodium azide
-
Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Handhabung
- Avoid repeated freezing and thawing.
-
Lagerung
- 4 °C/-20 °C
-
Informationen zur Lagerung
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
-
-
- HA-Tag
-
Andere Bezeichnung
- HA Epitope Tag (YPYDVPDYA)
-
Substanzklasse
- Tag
-
Hintergrund
- Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYAYPYDVPDYA) is an established example. This polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells.
Target
-