Nanog Antikörper

Produktnummer ABIN356386

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Nanog in WB, IHC (p) und EIA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Nanog Antikörper (ABIN356386)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nanog Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Klon: 60CT70-5-12

Produktdetails anti-Nanog Antikörper

Spezifität: This antibody is specific to Human NANOG.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Produktmerkmale: Synonyms: Homeobox protein NANOG

Aufreinigung: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from mouse immunized with human recombinant NANOGprotein.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: 1/1,000. Western blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: PBS, 0.09 % (W/V) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Andere Bezeichnung: NANOG

Hintergrund: NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cell proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.Synonyms: Homeobox protein NANOG

Molekulargewicht: 34620 Da

Gen-ID: 79923

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance