IL7R Antikörper (pTyr449)

Produktnummer ABIN351553

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch IL7R in WB, ELISA, IHC und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für IL7R Antikörper (pTyr449) (ABIN351553)

Target: IL7R (Interleukin 7 Receptor (IL7R))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL7R Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-IL7R Antikörper

Bindungsspezifität: pTyr449

Spezifität: A BLAST analysis was used to suggest cross-reactivity with IL-7R from human, mouse and rat sources based on 100 % homology with the immunizing sequence. Cross-reactivity with IL-7R from other sources has not been determined.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to residues near Y449 of mouse IL-7 protein.

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:100000), IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:10000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Expect a band approximately 55 kD in size corresponding to phosphorylated IL-7 in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with mouse IL-7 pY449 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu IL7R

Target: IL7R (Interleukin 7 Receptor (IL7R))

Andere Bezeichnung: IL7R / CD127

Hintergrund: Name/Gene ID: IL7R
Family: Interleukin

Synonyms: IL7R, CD127, CD127 antigen, IL-7RA, IL-7 receptor subunit alpha, IL7RA, ILRA, Interleukin 7 receptor, CDW127, IL-7R subunit alpha, IL-7R-alpha

Gen-ID: 3575

UniProt: P16871

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size