IL7R Antikörper (C-Term)

Produktnummer ABIN351552

Der Kaninchen Polyklonal anti-IL7R Antikörper (ABIN351552) detektiert spezifisch IL7R in WB, ELISA, IHC und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für IL7R Antikörper (C-Term) (ABIN351552)

Target: IL7R (Interleukin 7 Receptor (IL7R))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL7R Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-IL7R Antikörper

Bindungsspezifität: C-Term

Spezifität: A BLAST analysis was used to suggest cross-reactivity with IL-7 from human, mouse and rat sources based on 100 % homology with the immunizing sequence. Cross-reactivity with IL-7 from other sources has not been determined

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to a region near the carboxy terminus of mouse IL-7 protein.

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:500 - 1:50000), IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:10000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Expect a band approximately 55 kD in size corresponding to IL-7 in the appropriate cell lysate or extract.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles. Store undiluted.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Details zu IL7R

Target: IL7R (Interleukin 7 Receptor (IL7R))

Andere Bezeichnung: IL7R / CD127

Hintergrund: Name/Gene ID: IL7R
Family: Interleukin

Synonyms: IL7R, CD127, CD127 antigen, IL-7RA, IL-7 receptor subunit alpha, IL7RA, ILRA, Interleukin 7 receptor, CDW127, IL-7R subunit alpha, IL-7R-alpha

Gen-ID: 3575

UniProt: P16871

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size