PPARG Antikörper (AA 1-16)

Produktnummer ABIN351540

Dieses Anti-PPARG-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PPARG in WB, IHC, ICC, IHC (p) und GS. Geeignet für Human und Affe.

Kurzübersicht für PPARG Antikörper (AA 1-16) (ABIN351540)

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PPARG Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Gel Shift (GS)

Produktdetails anti-PPARG Antikörper

Bindungsspezifität: AA 1-16

Marke: IHC-plus™

Spezifität: Recognizes mouse PPARg2. Does not cross-react with PPARa or PPARd.

Homologie: Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset (100%) Gorilla, Cat, Bat, Dog (94%) Pig (88%) Elephant (81%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to aa1-16 (MGETLGDSPIDPESDS) of human PPARg2 (peroxisome proliferator activated receptor g2). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset (100%), Gorilla, Cat, Bat, Dog (94%), Pig (88%), Elephant (81%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: GS, ICC (1:500), IHC, IHC-P (2.5 - 5 μg/mL), WB (1:500)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5-5 μg/mL. Western Blot: 1:500. Detects a band of ~56kD.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 1 mg/mL BSA, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu PPARG

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Andere Bezeichnung: PPARG / PPAR Gamma

Hintergrund: Name/Gene ID: PPARG
Subfamily: NR1 Thyroid hormone-like
Family: NHR

Synonyms: PPARG, NR1C3, PPAR gamma, PPAR gamma 2, PPARG1, PPARG2, PPARgamma, CIMT1, GLM1, PPAR-gamma

Gen-ID: 5468

UniProt: P37231

Pathways: MAPK Signalweg, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation