ERK1/2 Antikörper (pThr202, pTyr204)

Produktnummer ABIN350736

Dieses Anti-ERK1/2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ERK1/2 in WB und IHC. Geeignet für Human.

Kurzübersicht für ERK1/2 Antikörper (pThr202, pTyr204) (ABIN350736)

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ERK1/2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-ERK1/2 Antikörper

Bindungsspezifität: pThr202, pTyr204

Verwendungszweck: Rabbit antibody to phospho T202 & Y204 Erk1 Erk2

Spezifität: Specific for Erk1 and Erk2.

Kreuzreaktivität: Human, Maus, Ratte

Kreuzreaktivität (Details): Other species not yet tested.

Aufreinigung: IgG

Immunogen: A synthetic peptide from the phospho T202 & Y204 of human Erk1 Erk2 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in many species including rat mouse zebra fish xenopus and chicken.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. This antibody may also recognise the unphosphorylated form. Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handhabung: Avoid freeze and thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Haltbarkeit: 12 months

Details zu ERK1/2

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Andere Bezeichnung: Erk1 Erk2

Hintergrund: FUNCTION: Involved in both the initiation and regulation of meiosis mitosis and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1, required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1. Phosphorylates heat shock factor protein 4 (HSF4). CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. COFACTOR: Magnesium. ENZYME REGULATION: Activated by tyrosine phosphorylation in response to insulin and NGF. SUBUNIT: Interacts with MORG1. Binds to HIV-1 Nef. This interaction inhibits its kinase activity. Interacts with HSF4 and NISCH.

UniProt: P27361