GAPDH Antikörper

Produktnummer ABIN350328

Zitiert in 1 Publikation. Dieser Anti-GAPDH-Antikörper ist ein Kaninchen-Polyklonal-Antikörper zum Nachweis von GAPDH in WB und IHC. Geeignet für Ratte.

Kurzübersicht für GAPDH Antikörper (ABIN350328)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GAPDH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-GAPDH Antikörper

Verwendungszweck: Rabbit antibody to GAPDH

Spezifität: Specific for GAPDH.

Kreuzreaktivität: Human, Marmoset, Maus, Ratte

Kreuzreaktivität (Details): Other species not yet tested.

Aufreinigung: IgG

Immunogen: A synthetic peptide from rat GAPDH conjugated to blue carrier protein was used as the antigen. The antigen is homologous in mouse and shares 90% identity with human sequence.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC WB. A concentration of 1-5,micro,g,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handhabung: Avoid freeze and thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-GAPDH Antikörper (ABIN350328)

Gengenbacher, Müller-Rudorf, Poggio, Gräßel, Dumit, Kreutmair, Lippert, Duyster, Illert: "Proteomic Phosphosite Analysis Identified Crucial NPM-ALK-Mediated NIPA Serine and Threonine Residues." in: International journal of molecular sciences, Vol. 20, Issue 16, (2020) (PubMed).

Details zu GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Andere Bezeichnung: GAPDH

Hintergrund: FUNCTION: May bind tRNA. Has uracil-DNA-glycosylase activity. May activate transcription in neurons. Functions as a killing protein in apoptosis when over-expressed. CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. PATHWAY: Carbohydrate degradation, glycolysis, pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: High levels in skeletal muscle and heart low levels in liver brain and kidney.

UniProt: P04797