Choline Acetyltransferase Antikörper

Produktnummer ABIN349982

Der Kaninchen Polyklonal Anti-Choline Acetyltransferase-Antikörper wurde für IHC validiert. Er ist geeignet, Choline Acetyltransferase in Proben von Maus zu detektieren.

Kurzübersicht für Choline Acetyltransferase Antikörper (ABIN349982)

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Choline Acetyltransferase Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Produktdetails anti-Choline Acetyltransferase Antikörper

Verwendungszweck: Rabbit antibody to ChAT

Spezifität: Specific for ChAT.

Kreuzreaktivität: Human, Maus, Ratte

Kreuzreaktivität (Details): Other species not yet tested.

Aufreinigung: IgG

Immunogen: A synthetic peptide from mouse ChAT conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and human.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC. A working concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handhabung: Avoid freeze and thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term.

Haltbarkeit: 12 months

Details zu Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Andere Bezeichnung: ChAT

Hintergrund: Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein. Choline acetylase catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine. Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2), also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy negative tests for anti-AChR antibodies and abrupt episodic crises with increased weakness bulbar paralysis and apnea precipitated by undue exertion fever or excitement. Inheritance is autosomal recessive.

UniProt: P28329

Pathways: Skeletal Muscle Fiber Development