ATG3 Antikörper

Produktnummer ABIN349972

Dieses Schaf Polyklonal-Antikörper erkennt spezifisch ATG3 in WB und IHC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für ATG3 Antikörper (ABIN349972)

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Reaktivität: Human

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser ATG3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-ATG3 Antikörper

Verwendungszweck: Sheep antibody to ATG3

Spezifität: Specific for ATG3.

Kreuzreaktivität: Human, Maus, Ratte

Kreuzreaktivität (Details): Other species not yet tested.

Aufreinigung: IgG

Immunogen: A synthetic peptide from human ATG3 conjugated to an immunogenic carrier protein has been used as the antigen. The antigen is homologous in rat and mouse.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC WB. Use at a concentration of 10-50,micro,g,ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handhabung: Avoid freeze and thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Haltbarkeit: 12 months

Details zu ATG3

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Andere Bezeichnung: ATG3

Hintergrund: Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3 a protein-conjugating enzyme essential for autophagy. FUNCTION: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy. SUBCELLULAR LOCATION: Cytoplasm TISSUE SPECIFICITY: Widely expressed with a highest expression in heart skeletal muscle kidney liver and placenta.

UniProt: Q9NT62

Pathways: Autophagie