GRP78 Antikörper

Produktnummer ABIN349665

Dieses Anti-GRP78-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von GRP78 in WB, ELISA, IF und EM. Geeignet für Chlamydomonas, Olive und Zea mays subsp. parviglumis (Balsas teosinte). Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für GRP78 Antikörper (ABIN349665)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reaktivität: Chlamydomonas, Olive, Zea mays subsp. parviglumis (Balsas teosinte)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GRP78 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Electron Microscopy (EM)

Produktdetails anti-GRP78 Antikörper

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 73.5 / 80 kDa

Aufreinigung: affinity purified

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 At5g28540 Q9LKR3, BiP2 At5g42020 F4K007 , BiP3 At5g09080 Q8H1B3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: 1 : 8000 (ELISA), 1 : 2000 with standard ECL (WB), 1 : 600 ( IF).

Kommentare:

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 200 µL of sterile water

Buffer: PBS pH 7.4

Handhabung: Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Lagerung: -20 °C

Referenzen für anti-GRP78 Antikörper (ABIN349665)

Suliman, Chateigner-Boutin, Francin-Allami, Partier, Bouchet, Salse, Pont, Marion, Rogniaux, Tessier, Guillon, Larré: "Identification of glycosyltransferases involved in cell wall synthesis of wheat endosperm." in: Journal of proteomics, Vol. 78, pp. 508-21, (2013) (PubMed).

Sikorskaite, Rajamäki, Baniulis, Stanys, Valkonen: "Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families." in: Plant methods, Vol. 9, Issue 1, pp. 31, (2013) (PubMed).

Details zu GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Andere Bezeichnung: BiP

Hintergrund: AGI Code: At5g42020, At5g42020
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).

Molekulargewicht: expected: 73.5 kDa, apparent: 80 kDa

UniProt: Q39043

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling