GRP78 Antikörper

Produktnummer ABIN349665

Zitiert in 2+ Publikationen. Der Kaninchen Polyklonal anti-GRP78 Antikörper (ABIN349665) detektiert spezifisch GRP78 in WB, ELISA, IF und EM. Dieser Antikörper reagiert spezifisch mit Proben aus Spinach, Arabidopsis thaliana, Mais (Zea mays), Physcomytrium Patens, Cashew nut, Tomato, Triticum aestivum, Olea europaea, Chlamydomonas reinhardtii, Cucumis sativus, Mangifera indica, Moniliophthora perniciosa, Nicotiana benthamiana, Nicotiana tabacum, Chara australis, Oryza sativa, Picea abies, Pistachio, Potato, Radieschen, Rape und Schinus molle.

Kurzübersicht für GRP78 Antikörper (ABIN349665)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reaktivität: Spinach, Arabidopsis thaliana, Mais (Zea mays), Physcomytrium Patens, Cashew nut, Tomato, Triticum aestivum, Olea europaea, Chlamydomonas reinhardtii, Cucumis sativus, Mangifera indica, Moniliophthora perniciosa, Nicotiana benthamiana, Nicotiana tabacum, Chara australis, Oryza sativa, Picea abies, Pistachio, Potato, Radieschen, Rape, Schinus molle

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GRP78 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Electron Microscopy (EM)

Produktdetails anti-GRP78 Antikörper

Verwendungszweck: Anti-BiP | Lumenal-binding protein (rabbit antibody)

Kreuzreaktivität (Details): Anacardium occidentale, Arabidopsis thaliana, Brassica napus,Chara australis R.Br, Chlamydomonas reinhardtii, Cucumis sativus, Mangifera indica, Moniliophthora perniciosa, Nicotiana benthamiana, Nicotiana tabacum, Raphanus sativa L. Tokinashi-daikon, Olea europaea, Oryza sativa, Picea abies, Pistachio sp., Physcomitrium patens, Schinus molle, Spinacia oleracea, Solanum lycopersicum, Solanum tuberosum, Triticum aestivum, Zea mays
Not reactive in: Ostreococcus tauri, Schizosaccharomyces pombe

Homologie: Arabis alpina,Capsella rubella,Capsicum annuum,Citrus clementina,Citrus sinsensis,Eucalyptus grandis,Glycine max,Hordeum vulgare,Isatis tincorina,Prunus persica,Triticum aestivium,Petunia hybrida,Picea sitcHensis,Populus trichocarpa,Ricinus comminus,Vitis vinifera

Aufreinigung: Immunogen affinity purified serum

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 At5g28540 Q9LKR3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: 1 : 8000 (ELISA), 1 : 600 (IF), 1 : 2000 (WB)

Kommentare:

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Referenzen für anti-GRP78 Antikörper (ABIN349665)

Suliman, Chateigner-Boutin, Francin-Allami, Partier, Bouchet, Salse, Pont, Marion, Rogniaux, Tessier, Guillon, Larré: "Identification of glycosyltransferases involved in cell wall synthesis of wheat endosperm." in: Journal of proteomics, Vol. 78, pp. 508-21, (2013) (PubMed).

Sikorskaite, Rajamäki, Baniulis, Stanys, Valkonen: "Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families." in: Plant methods, Vol. 9, Issue 1, pp. 31, (2013) (PubMed).

Details zu GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Andere Bezeichnung: BiP

Hintergrund: BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).

Molekulargewicht: 73.5 | 80 kDa

Gen-ID: 834207

UniProt: Q39043

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling