COL2 Antikörper

Produktnummer ABIN344746

Dieses Maus Monoklonal-Antikörper erkennt spezifisch COL2 in WB, IHC, ELISA und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus und Huhn.

Kurzübersicht für COL2 Antikörper (ABIN344746)

Target: COL2 (Collagen, Type II (COL2))

Reaktivität: Human, Maus, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser COL2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-COL2 Antikörper

Spezifität: Recognizes both alpha 1 (II) and alpha 3 (XI) chains which have identical primary structure. It also reacts with pepsin-digested type II collagen. Its epitope is localized in the triple helix of type II collagen. It shows no cross-reaction with type I or type III collagen. Species cross-reactivity: Human, Chicken, Mouse, Salamander.

Aufreinigung: Purified

Immunogen: Purified type II collagen, obtained from adult chicken sternum by limited pepsinization.

Type of Immunogen: Purified protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-P (1:200), WB (1:200 - 1:400)

Usage: In Western blotting, antibody reacts with the TCA fragment of lathyritic type II collagen after digestion with mammalian collagenase. Western Blot of CNBr peptides of collagen II shows that this antibody reacts with CB11 fragment which is the site of immunogenic and arthritogenic epitopes along the intact type II molecule. ELISA. Western Blot: 1:200-1:400. Cartilage is an appropriate positive control. Immunohistochemistry (Formalin/Paraffin): 1-2 μg/mL. Staining of paraffin embedded tissues requires digestion of tissue sections with pepsin at 1 mg/mL Tris HCl,  pH 2 for 15 minutes at RT or 15 minutes at 37?C.

Kommentare:

Target Species of Antibody: Chicken

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 10 mM PBS,  pH 7.4, 0.2 % BSA, 15 mM sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu COL2

Target: COL2 (Collagen, Type II (COL2))

Andere Bezeichnung: Collagen Type II