Phosphothreonine-PROLINE Antikörper

Produktnummer ABIN344517

Der Maus Monoklonal anti-Phosphothreonine-PROLINE Antikörper (ABIN344517) detektiert spezifisch Phosphothreonine-PROLINE in WB, ELISA, IHC und IHC (p).

Kurzübersicht für Phosphothreonine-PROLINE Antikörper (ABIN344517)

Target: Phosphothreonine-PROLINE

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails

Spezifität: Detects phospho- threonine only when followed by proline. It reacts with proteins/peptides phosphorylated on the Thr-Pro motif in an otherwise highly context-independent fashion. The phosphospecific antibody does not recognize phospho-threonine in the absence of an adjacent proline. Does not react with phospho-tyrosine, but does react with some phospho-serine peptides containing the phospho-serine-proline motif (e. g., phospho-Elk-1). Recognizes all species.

Aufreinigung: Protein A purified

Immunogen: Synthetic phosphothreonine- proline-containing peptides (KLH coupled). Antibody is a mouse IgM clone and can be recognized by anti-mouse Ig (whole molecule) secondary antibody.

Type of Immunogen: Synthetic peptide

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:1000), IHC, IHC-P, WB (1:5000)

Usage: Western Blot: 1:5000. Immunohistochemistry (Paraffin): 1:50. ELISA: 1:1000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Konservierungsmittel: Without preservative

Antigendetails

Target: Phosphothreonine-PROLINE

Andere Bezeichnung: Phospho-Threonine-Proline

Substanzklasse: Chemical