Der Kaninchen Polyklonal anti-Phosphothreonine-PROLINE Antikörper wird verwendet zum Nachweis von Phosphothreonine-PROLINE. Er wurde validiert für WB und ELISA.
Kurzübersicht für Phosphothreonine-PROLINE Antikörper (ABIN285914)
Target
Phosphothreonine-PROLINE
Wirt
Kaninchen
Klonalität
Polyklonal
Konjugat
Unkonjugiert
Applikation
Western Blotting (WB), ELISA
Spezifität
Recognizes protein containing phosthronine-proline motif, also reacts to phosphoserine-prolin motif to a similar degree. The antibodies do not react to phosphothreonie, phosphoserine or phsophotyrosine under optimal conditions.0.02% NaN3
Immunogen
Phosphothreonine-Pro antibody was raised in rabbit using KLH-phosphothreonine-proline amine (pT-P-NH2) peptide conjugate as the immunogen.
ELISA, 0.5 µg/mL, WB: 2 µg/mL Optimal conditions should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
Lot specific
Buffer
PBS with 0.2 % NaN3.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handhabung
DO NOT FREEZE. Dilute only prior to immediate use.
Lagerung
4 °C
Target
Phosphothreonine-PROLINE
Substanzklasse
Chemical
Hintergrund
Threonine is one of the 20 naturally occurring amino acids. Kinases phosphorylate threonine to phosphothreonine which enters various biosynthetic pathways in the body. The threonine residue is susceptible to numerous posttranslational modifications. The hydroxy side-chain can undergo O-linked glycosylation. In addition, threonine residues undergo phosphorylation through the action of a threonine kinase. In its phosphorylated form, it can be referred to as phosphothreonine.