Ubiquitin Antikörper

Produktnummer ABIN340405

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Ubiquitin in WB, IHC, IF, IHC (p), IP und IHC (fro). Er zeigt eine Reaktivität gegenüber Human und Rind (Kuh).

Kurzübersicht für Ubiquitin Antikörper (ABIN340405)

Target: Ubiquitin (Ub)

Reaktivität: Human, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Ubiquitin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Ubiquitin Antikörper

Spezifität: Recognizes ubiquitin, both conjugated and unconjugated. Reactivity has been shown in human, bovine, chicken, Drosophila and C. elegans.

Aufreinigung: Purified

Immunogen: Purified bovine ubiquitin conjugated with glutaraldehyde to keyhole limpet hemocyanin (KLH).

Type of Immunogen: Hapten

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: IF (1:10), IHC, IHC-Fr, IHC-P, IP, WB (1:10)

Usage: Can be used for immunoassays, Western Blot, Immunofluorescence and Immunoprecipitation. Furthermore the antibody is useful for Immunohistology after heat-induced retrieval on both frozen and paraffin embedded sections. Immunohistology: 1:10. Immunofluorescence: 1:10. Western Blot: 1:10. For Western Blot it is recommended to use lysis buffer in 10 mM N-ethylmaleimide to inhibit ubiquitin-conjugating enzymes. N-ethylmaleimide inactivates certain enzymes by blocking free sulfhydryls. After electrophoresis and transfer, pre-incubate transferred membranes in denaturing buffer (6M guanidine-HCl, 20 mM Tris-HCl, 5 mM beta-mercaptoethanol, 1 mM PMSF (  pH 7.5)) for 30-60 minutes at 4?C, followed by extensive PBS washing.

Kommentare:

Target Species of Antibody: Bovine

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.02 % sodium azide, 0.1 % BSA.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu Ubiquitin

Target: Ubiquitin (Ub)

Andere Bezeichnung: Ubiquitin

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway