Iba1 Antikörper (AA 149-161)

Produktnummer ABIN337364

Der Ziege Polyklonal anti-Iba1 Antikörper wird verwendet zum Nachweis von Iba1 in Proben von Human, Ratte, Maus, Schwein, Affe und Hamster. Er wurde validiert für WB, IHC, ELISA und IHC (p).

Kurzübersicht für Iba1 Antikörper (AA 149-161) (ABIN337364)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reaktivität: Human, Ratte, Maus, Schwein, Affe, Hamster

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Iba1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Iba1 Antikörper

Bindungsspezifität: AA 149-161

Spezifität: Human AIF1 / IBA1. This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Pig (100%) Goat, Elephant, Bovine, Horse, Rabbit, Platypus (92%) Panda, Dog, Bat (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-TGPPAKKAISELP from the C-terminus of human AIF1 / IBA1 (NP_116573.1, NP_001614.3). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Pig (100%), Goat, Elephant, Bovine, Horse, Rabbit, Platypus (92%), Panda, Dog, Bat (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:128000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Andere Bezeichnung: AIF1 / IBA1

Hintergrund: Name/Gene ID: AIF1

Synonyms: AIF1, AIF-1, Em:AF129756.17, G1, IRT-1, Protein G1, IBA1, IRT1

Gen-ID: 199

NCBI Accession: NP_116573, NP_001614

UniProt: P55008

Pathways: Smooth Muscle Cell Migration