MMP2 Antikörper (AA 468-483)

Produktnummer ABIN337360

Dieses Maus Monoklonal-Antikörper erkennt spezifisch MMP2 in WB, IHC, ELISA, IHC (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human und Schwein.

Kurzübersicht für MMP2 Antikörper (AA 468-483) (ABIN337360)

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Reaktivität: Human, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MMP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-MMP2 Antikörper

Bindungsspezifität: AA 468-483

Marke: IHC-plus™

Spezifität: Recognizes the precursor and active forms of human MMP-2. Species cross-reactivity: mouse, rat, rabbit, porcine, bovine and equine MMP-2, but not with human MMP-1, 3, 9 and 13.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Pig (100%) Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Panda, Dog, Rabbit, Horse (94%) Bovine (88%) Chicken (81%).

Aufreinigung: Purified

Immunogen: Synthetic oligopeptide corresponding to aa residues 468-483 (VTPRDKPMGPLLVATF) of human MMP-2. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Pig (100%), Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Panda, Dog, Rabbit, Horse (94%), Bovine (88%), Chicken (81%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-Fr (0.9 μg/mL), IHC-P (10 μg/mL), WB (1 - 5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.1 M PBS,  pH 7.0, 2 % BSA (protease free).

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Details zu MMP2

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Andere Bezeichnung: MMP2 / MMP-2

Hintergrund: Name/Gene ID: MMP2
Subfamily: Metallopeptidase M10A
Family: Protease

Synonyms: MMP2, 72 kd type iv collagenase, 72 kDa gelatinase, 72 kd gelatinase, 72 kDa type IV collagenase, CLG4A, CLG4, GelA, Gelatinase A, Matrix metalloproteinase-2, MMP-2, MONA, Neutrophil gelatinase, TBE-1, Tbe-1 metalloprotease, Collagenase type IV-A, Matrix metalloproteinase-II, MMP-II

Gen-ID: 4313

UniProt: P08253

Pathways: Activation of Innate immune Response