CAMK2B Antikörper (pThr286)

Produktnummer ABIN337356

Der Kaninchen Polyklonal anti-CAMK2B Antikörper (ABIN337356) detektiert spezifisch CAMK2B in WB, IHC und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte, Maus und Xenopus laevis.

Kurzübersicht für CAMK2B Antikörper (pThr286) (ABIN337356)

Target: CAMK2B (Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B))

Reaktivität: Human, Ratte, Maus, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CAMK2B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CAMK2B Antikörper

Bindungsspezifität: pThr286

Spezifität: Specific for the ~50k a-CaM Kinase II and the ~60k R-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is blocked by the X-phosphatase treatment

Aufreinigung: Immunoaffinity purified

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phosphoThr286 found in rat brain CaM Kinase II.

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (1:200), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:200.

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA, 50 % glycerol

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu CAMK2B

Target: CAMK2B (Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B))

Andere Bezeichnung: CAMK2 / CAMKII

Hintergrund: Name/Gene ID: CAMK2

Synonyms: CAMK2

Pathways: WNT Signalweg, Interferon-gamma Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity