Thrombospondin 1 Antikörper (Collagen-binding Domain)

Produktnummer ABIN337292

Der Maus Monoklonal Anti-Thrombospondin 1-Antikörper wurde für WB, IHC, IP, IF, FACS und IHC (p) validiert. Er ist geeignet, Thrombospondin 1 in Proben von Human, Maus, Ratte, Rind (Kuh), Pferd, Schwein, Hund und Schaf zu detektieren.

Kurzübersicht für Thrombospondin 1 Antikörper (Collagen-binding Domain) (ABIN337292)

Target: Thrombospondin 1 (THBS1)

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Pferd, Schwein, Hund, Schaf

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Thrombospondin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: A6-1

Produktdetails anti-Thrombospondin 1 Antikörper

Bindungsspezifität: Collagen-binding Domain

Marke: IHC-plus™

Spezifität: Recognizes Thrombospondin. Shows no cross-reactivity with fibronectin, fibrinogen and von Willebrand factor. Shows a mild cross-reaction with TSP 2. Inhibits TSP-collagen interaction. Antibody binding to TSP is unaffected by glycosaminoglycans (e. g. hyaluronic acid, chondroitin sulfate and heparin). Its binding is enhanced by EDTA i. e. at low concentration of Ca2+. Species cross-reactivity: Human, bovine, porcine, equine, canine, sheep, mouse and rat.

Aufreinigung: Protein G purified

Immunogen: Reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets. MW of Antigen: ~450kD (non-reduced), 170-180kD (reduced). Epitope: Collagen Type V-binding domain of TSP.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: Flo, IF, IHC, IHC-P (10 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4.

Konservierungsmittel: Azide free

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Thrombospondin 1

Target: Thrombospondin 1 (THBS1)

Andere Bezeichnung: Thrombospondin 1

Pathways: Autophagie