GJC1 Antikörper (AA 354-367)

Produktnummer ABIN337289

Der Maus Monoklonal Anti-GJC1-Antikörper wurde für WB, IHC, IHC (p) und ICC validiert. Er ist geeignet, GJC1 in Proben von Human, Ratte, Maus und Hund zu detektieren.

Kurzübersicht für GJC1 Antikörper (AA 354-367) (ABIN337289)

Target: GJC1 (Gap Junction Protein, gamma 1, 45kDa (GJC1))

Reaktivität: Human, Ratte, Maus, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GJC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Produktdetails anti-GJC1 Antikörper

Bindungsspezifität: AA 354-367

Marke: IHC-plus™

Spezifität: Recognizes human Connexin 45. Species cross-reactivity: rat. Species sequence homology: canine: 100 % and mouse: 92 %.

Aufreinigung: Protein A purified

Immunogen: Synthetic peptide corresponding to aa354-367 of human connexin 45. (H-Gln-Ala-Tyr-Ser-His-Gln-Asn-Asn-Pro-His-Gly-Pro-Arg-Glu- Cys-OH) (KLH).

Type of Immunogen: Synthetic peptide

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.6, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu GJC1

Target: GJC1 (Gap Junction Protein, gamma 1, 45kDa (GJC1))

Andere Bezeichnung: GJC1 / CX45 / Connexin 45

Hintergrund: Name/Gene ID: GJC1
Subfamily: Connexin
Family: Ion Channel

Synonyms: GJC1, Connexin-45, Connexin 45, Gap junction alpha-7 protein, Gap junction gamma-1 protein, CX45, Gap junction protein alpha-6, GJA7

Gen-ID: 10052

UniProt: P36383

Pathways: Cell-Cell Junction Organization