GJC1 Antikörper (Intracellular)

Produktnummer ABIN7886193

Der Kaninchen Polyklonal anti-GJC1 Antikörper wird verwendet zum Nachweis von GJC1 in Proben von Ratte. Er wurde validiert für WB, IHC und IF.

Kurzübersicht für GJC1 Antikörper (Intracellular) (ABIN7886193)

Target: GJC1 (Gap Junction Protein, gamma 1, 45kDa (GJC1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GJC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-GJC1 Antikörper

Bindungsspezifität: AA 153-166, Intracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to Connexin-45

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse,human - identical

Produktmerkmale: Anti-Connexin-45 (GJC1) Antibody is directed against an epitope of rat Connexin-45. Anti-Connexin-45 (GJC1) Antibody (ABIN7043207, ABIN7044085 and ABIN7044086) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Cx45 from human, rat and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)KEQSQPKPKHDGRR, corresponding to amino acid residues 153-166 of rat Connexin-45

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:400

IP: The optimal concentration should be determined by the user

Kommentare:

Cited Application: IP

Negative Control: (ABIN7235558)

Blocking Peptide: (ABIN7235558)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu GJC1

Target: GJC1 (Gap Junction Protein, gamma 1, 45kDa (GJC1))

Andere Bezeichnung: GJC1

Hintergrund:

Synonyms: CX45, Gap junction γ1 protein, Gap junction α7 protein, GJA7

Description: Gap junctions are plasma membrane domains of intercellular channels that allow direct connection between the cytoplasm of adjacent cells. They allow exchange of low molecular weight metabolites, ions and small hydrophilic molecules. These channels are formed by head-to-head docking of hexameric hemichannels called connexons, whose subunit proteins are encoded by the connexin (Cx) gene family1-3.The connexin subunit is formed of four-transmembrane spanning domains, harboring two extracellular loops, one cytoplasmic loop and cytoplasmic N- and C-terminal regions. To date, the family of connexin comprises 21 genes in the human genome4.Connexin 45 (Cx45), encoded by the GJC1 gene, is expressed in cardiomyocytes and in developing vasculature in both endothelial and smooth muscle cells4,5.In the heart, Cx45 along with Cx40 and Cx43, play a crucial role in normal electrical conduction and synchronous heart muscle contraction and important for the normal embryonic development of the heart6. Indeed Cx45 forms low conductance and voltage-sensitive gap junctions between the ventricular cardiac myocytes and the core conduction system2. Studies have showed that cardiac connexin Cx45 is upregulated in human failing hearts and has an important role in the regulation of endothelial-induced mural cell differentiation4,5.

Gen-ID: 266706

NCBI Accession: NM_005497

UniProt: A4GG66

Pathways: Cell-Cell Junction Organization