COPA Antikörper (AA 1-19)

Produktnummer ABIN337288

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch COPA in WB, IHC, ICC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Hund, Zebrafisch (Danio rerio), Pferd, Rind (Kuh), Fledermaus, Huhn, Hamster, Affe, Schwein und Xenopus laevis.

Kurzübersicht für COPA Antikörper (AA 1-19) (ABIN337288)

Target: COPA (Coatomer Protein Complex, Subunit alpha (COPA))

Reaktivität: Human, Maus, Ratte, Hund, Zebrafisch (Danio rerio), Pferd, Rind (Kuh), Fledermaus, Huhn, Hamster, Affe, Schwein, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser COPA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-COPA Antikörper

Bindungsspezifität: AA 1-19

Marke: IHC-plus™

Spezifität: Detects rat coatomer-protein I alpha (COP I alpha). Species cross-reactivity: Human.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bat, Bovine, Dog, Horse, Pig, Opossum, Chicken, Xenopus, Salmon, Stickleback, Pufferfish, Zebrafish, Seq squirt, Ant, Water flea, Tick (100%) Mosquito, Sea anemone, Rice, Beetle, Arabidopsis, Spikemoss (94%) Drosophila, Trichoplax, Grape, Castor bean (89%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide: M(1)LTKFETKSARVKGLSFHP(19)C. Immunizing peptide corresponds to amino acid residues 1-19 from rat COP I alpha. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bat, Bovine, Dog, Horse, Pig, Opossum, Chicken, Xenopus, Salmon, Stickleback, Pufferfish, Zebrafish, Seq squirt, Ant, Water flea, Tick (100%), Mosquito, Sea anemone, Rice, Beetle, Arabidopsis, Spikemoss (94%), Drosophila, Trichoplax, Grape, Castor bean (89%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 1 μg/mL, detects an ~140kD protein representing COPI alpha from PC-12 cell extract. Also detects an unidentified protein at ~100kD.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu COPA

Target: COPA (Coatomer Protein Complex, Subunit alpha (COPA))

Andere Bezeichnung: COPA / Xenin

Hintergrund: Name/Gene ID: COPA

Synonyms: COPA, Alpha coat protein, Alpha-COP, Alpha COP I, Alpha-coat protein, Coatomer alpha subunit, Coatomer subunit alpha, HEPCOP, HEP-COP, Xenin

Gen-ID: 1314

UniProt: P53621

Pathways: Hormone Activity