OGT Antikörper (AA 543-555)

Produktnummer ABIN337257

Der Ziege Polyklonal anti-OGT Antikörper (ABIN337257) detektiert spezifisch OGT in WB, ELISA, IHC und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Hund, Rind (Kuh), Pferd, Kaninchen, Fledermaus, Affe, Schwein und Hamster.

Kurzübersicht für OGT Antikörper (AA 543-555) (ABIN337257)

Target: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Pferd, Kaninchen, Fledermaus, Affe, Schwein, Hamster

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser OGT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-OGT Antikörper

Bindungsspezifität: AA 543-555

Spezifität: Human OGT. This antibody is expected to recognise both reported isoforms (NP_858058.1 and NP_858059.1

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig (100%) Xenopus (92%) Turkey, Chicken, Pufferfish, Zebrafish (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-YEHPKDLKLSDGR from an internal region of human OGT (NP_858058.1, NP_858059.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig (100%), Xenopus (92%), Turkey, Chicken, Pufferfish, Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:64000), WB (1:64000, 0.05 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu OGT

Target: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Andere Bezeichnung: OGT / O-GLCNAC

Hintergrund: Name/Gene ID: OGT

Synonyms: OGT, HRNT1, O-GLCNAC

Gen-ID: 8473

NCBI Accession: NP_858058, NP_858059

UniProt: O15294

Pathways: Regulation of Carbohydrate Metabolic Process