OGT Antikörper (N-Term)

Produktnummer ABIN6972463

Produktdetails anti-OGT Antikörper

Target: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser OGT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Produktmerkmale: OGT (O-linked N-acetylglucosamine (GlcNAc) transferase) catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to serine or threonine residues in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). OGT can glycosylate a large and diverse number of proteins including HCF1, AKT1, MLL5 and histone H2B. It can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Specifically OGT has been shown to interact directly with TET2 and TET3, enzymes that catalyze the oxidation of 5-methylcytosine on DNA. The TET/OGT interaction leads to GlcNAcylation of HCF1, a protein component of the COMPASS complex, which regulates H3K4 methylation and gene expression. OGT/O-GlcNAc transferase antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq and Western blot, it has been shown to react with Human and Mouse samples.

Aufreinigung: Affinity Purified

Immunogen: This antibody was raised against a recombinant protein corresponding to the N-terminal region of human OGT.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Details zu OGT

Target: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Andere Bezeichnung: OGT/O-GlcNAc transferase (OGT Produkte)

Synonyme: BcDNA:GH04245 antikoerper, CG10392 antikoerper, Dmel\\CG10392 antikoerper, OGT antikoerper, Ogt antikoerper, P1201 antikoerper, SXC antikoerper, Sxc antikoerper, l(2)02637 antikoerper, l(2)NC130 antikoerper, sxc/Ogt antikoerper, fm81g08 antikoerper, ogt antikoerper, wu:fc12b01 antikoerper, wu:fm81g08 antikoerper, HRNT1 antikoerper, O-GLCNAC antikoerper, 1110038P24Rik antikoerper, 4831420N21Rik antikoerper, AI115525 antikoerper, Ogtl antikoerper, O-linked N-acetylglucosamine (GlcNAc) transferase L homeolog antikoerper, super sex combs antikoerper, O-linked N-acetylglucosamine (GlcNAc) transferase, tandem duplicate 1 antikoerper, O-linked N-acetylglucosamine (GlcNAc) transferase antikoerper, O-linked GlcNAc transferase antikoerper, o-linked GlcNAc transferase antikoerper, O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) antikoerper, ogt.L antikoerper, sxc antikoerper, ogt.1 antikoerper, OGT antikoerper, ogt antikoerper, GL50803_12081 antikoerper, GL50803_41701 antikoerper, THAPSDRAFT_264441 antikoerper, spyA antikoerper, Ogt antikoerper

Molekulargewicht: 120 kDa

NCBI Accession: NP_858058

Pathways: Regulation of Carbohydrate Metabolic Process