HSPG2 Antikörper

Produktnummer ABIN337194

Dieses Anti-HSPG2-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von HSPG2 in WB, IHC, ELISA, IP, IHC (p) und IHC (fro). Geeignet für Human und Rind (Kuh).

Kurzübersicht für HSPG2 Antikörper (ABIN337194)

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Reaktivität: Human, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HSPG2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: A71

Produktdetails anti-HSPG2 Antikörper

Marke: IHC-plus™

Spezifität: Highly specific for bovine perlecan. The epitope is located in domain I. No cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin). Species cross-reactivity: Human.

Aufreinigung: Protein A/G purified

Immunogen: Bovine corneal endothelial cells.

Type of Immunogen: Cells

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:4000), IHC, IHC-Fr, IHC-P (5 μg/mL), IP, WB (1:50)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Bovine

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 15  mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu HSPG2

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Andere Bezeichnung: HSPG2 / Perlecan

Hintergrund: Name/Gene ID: HSPG2

Synonyms: HSPG2, Endorepellin (domain V region), HSPG, Heparan sulfate proteoglycan 2, Perlecan, Perlecan proteoglycan, SJA, SJS1, PRCAN, SJS

Gen-ID: 3339

UniProt: P98160

Pathways: Glycosaminoglycan Metabolic Process, Lipid Metabolism