JAG1 Antikörper (AA 110-125)

Produktnummer ABIN337168

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch JAG1 in WB, IHC, ELISA, ICC, IF und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.Es wurde in 1 Publikation zitiert.

Kurzübersicht für JAG1 Antikörper (AA 110-125) (ABIN337168)

Target: JAG1 (Jagged 1 (JAG1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser JAG1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-JAG1 Antikörper

Bindungsspezifität: AA 110-125

Spezifität: Recognizes human Jagged1 (JAG1).

Aufreinigung: Protein A purified

Immunogen: Synthetic peptide corresponding to aa 110-125 of human Jagged-1protein.

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:2500 - 1:10000), ICC, IF (1:100 - 1:500), IHC, IHC-P (5 μg/mL), WB (1:250 - 1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Referenzen für anti-JAG1 Antikörper (ABIN337168)

Siar, Ha, Aung, Nakano, Tsujigiwa, Nagatsuka, Ng, Kawakami: "Immunolocalization of notch signaling protein molecules in a maxillary chondrosarcoma and its recurrent tumor." in: European journal of medical research, Vol. 15, Issue 10, pp. 456-60, (2010) (PubMed).

Details zu JAG1

Target: JAG1 (Jagged 1 (JAG1))

Andere Bezeichnung: JAG1 / Jagged 1

Hintergrund: Name/Gene ID: JAG1

Synonyms: JAG1, AGS, AHD, Alagille syndrome, CD339 antigen, HJ1, Jagged1, JAGL1, Jagged, Jagged 1, Protein jagged-1, AWS, CD339, Soluble protein jagged

Gen-ID: 182

UniProt: P78504

Pathways: Notch Signalweg, Stem Cell Maintenance