ORAI3 Antikörper (N-Term)

Produktnummer ABIN337113

Der Kaninchen Polyklonal anti-ORAI3 Antikörper (ABIN337113) detektiert spezifisch ORAI3 in WB, IHC und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für ORAI3 Antikörper (N-Term) (ABIN337113)

Target: ORAI3 (ORAI Calcium Release-Activated Calcium Modulator 3 (ORAI3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ORAI3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ORAI3 Antikörper

Bindungsspezifität: N-Term

Spezifität: Recognizes human ORAI3, also known as Transmembrane protein 142C (TMEM142C), a 31.5 kD multi-pass membrane protein belonging to the ORAI family. Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. ORAI3 is one of two mammalian homologs of ORAI1, a four- transmembrane spanning protein that is an essential component of CRAC channels. ORAI3, along with ORAI1 and ORAI2, functions as a Ca2+ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+ sensor. Studies indicate that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. AHP1496 is not expected to cross react with either ORAI1 or ORAI2.

Aufreinigung: Affinity purified

Immunogen: A 15 amino acid peptide from near the amino terminus of human ORAI3.

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: LS-B2385 detects a band of approximately 35kD in A20 cell lysates.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Store undiluted.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles. Store undiluted.

Details zu ORAI3

Target: ORAI3 (ORAI Calcium Release-Activated Calcium Modulator 3 (ORAI3))

Andere Bezeichnung: ORAI3

Hintergrund: Name/Gene ID: ORAI3

Synonyms: ORAI3, Protein orai-3, TMEM142C, Transmembrane protein 142C

Gen-ID: 93129

UniProt: Q9BRQ5