NOTCH3 Antikörper (AA 2306-2318)

Produktnummer ABIN337107

Der Ziege Polyklonal anti-NOTCH3 Antikörper (ABIN337107) detektiert spezifisch NOTCH3 in ELISA, IHC und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Affe und Fledermaus.

Kurzübersicht für NOTCH3 Antikörper (AA 2306-2318) (ABIN337107)

Target: NOTCH3 (Notch 3 (NOTCH3))

Reaktivität: Human, Maus, Ratte, Affe, Fledermaus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NOTCH3 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-NOTCH3 Antikörper

Bindungsspezifität: AA 2306-2318

Spezifität: Human NOTCH3.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Panda, Bat (100%) Hamster, Bovine (92%) Elephant (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-QLGPQPEVTPKRQ from the C-terminus of human NOTCH3 (NP_000426.2). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Panda, Bat (100%), Hamster, Bovine (92%), Elephant (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:16000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu NOTCH3

Target: NOTCH3 (Notch 3 (NOTCH3))

Andere Bezeichnung: NOTCH3

Hintergrund: Name/Gene ID: NOTCH3
Family: Fibronectin

Synonyms: NOTCH3, CASIL, CADASIL, Notch 3, Notch (Drosophila) homolog 3, Notch homolog 3, Notch homolog 3 (Drosophila)

Gen-ID: 4854

NCBI Accession: NP_000426

UniProt: Q9UM47

Pathways: Notch Signalweg