NCF1 Antikörper (AA 378-390)

Produktnummer ABIN337093

Dieser Ziege Polyklonal Antikörper detektiert spezifisch NCF1 in WB, IHC, ELISA, IF und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Affe und Rind (Kuh).

Kurzübersicht für NCF1 Antikörper (AA 378-390) (ABIN337093)

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Reaktivität: Human, Affe, Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NCF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-NCF1 Antikörper

Bindungsspezifität: AA 378-390

Spezifität: Human NCF1 / p47phox.

Homologie: Percent identity by BLAST analysis: Human, Monkey, Marmoset, Dolphin, Elephant, Panda, Bovine (100%) Mouse, Rat (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-SESTKRKLASAV from the C-terminus of human NCF1 / p47phox (NP_000256.3). Percent identity by BLAST analysis: Human, Monkey, Marmoset, Dolphin, Elephant, Panda, Bovine (100%), Mouse, Rat (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IF, IHC, IHC-P (5 μg/mL), Peptide-ELISA, WB (0.2 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu NCF1

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Andere Bezeichnung: NCF1 / p47phox / p47 Phox

Hintergrund: Name/Gene ID: NCF1

Synonyms: NCF1, NCF1A, Neutrophil cytosolic factor 1, NCF-47K, Nox organizer 2, NOXO2, p47-phox, p47phox, SH3PXD1A, NADPH oxidase organizer 2, NCF-1, Neutrophil cytosol factor 1, Nox-organizing protein 2

Gen-ID: 653361

NCBI Accession: NP_000256

UniProt: P14598

Pathways: PI3K-Akt Signalweg