SRSF1 Antikörper (N-Term)

Produktnummer ABIN337068

This anti-SRSF1 antibody (ABIN337068) is a Mouse Monoclonal antibody detecting SRSF1 in WB, IHC, IP, IF, IHC (p). Suitable for Human, Mouse, Rat.

Kurzübersicht für SRSF1 Antikörper (N-Term) (ABIN337068)

Target: SRSF1 (serine/arginine-Rich Splicing Factor 1 (SRSF1))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SRSF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SRSF1 Antikörper

Bindungsspezifität: N-Term

Marke: IHC-plus™

Spezifität: Recognizes the N-terminal portion (aa1-97) of the SF2/ASF protein comprising the RNA-recognition motif (RRM1). Reactivity is not affected by phosphorylation state of SF2/ASF. Species cross-reactivity: Human (HeLa cells), mouse and rat. Cross-reactivity expected with other species because SF2/ASF is highly conserved.

Aufreinigung: Protein A purified

Immunogen: Recombinant SF2/ASF protein.

Type of Immunogen: Recombinant protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Approved: IF, IHC, IHC-P (5 μg/mL), IP, WB (0.5 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu SRSF1

Target: SRSF1 (serine/arginine-Rich Splicing Factor 1 (SRSF1))

Andere Bezeichnung: SFRS1 / SF2

Hintergrund: Name/Gene ID: SRSF1

Synonyms: SRSF1, Alternative-splicing factor 1, ASF, SF2, SF2p33, SFRS1, Splicing factor 2, ASF-1, SR splicing factor 1, SRp30a

Gen-ID: 6426

UniProt: Q07955

Pathways: Ribonucleoprotein Complex Subunit Organization