Isocitrate Dehydrogenase Antikörper

Produktnummer ABIN337057

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch Isocitrate Dehydrogenase in WB, ELISA, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Schwein und Vicia faba.

Kurzübersicht für Isocitrate Dehydrogenase Antikörper (ABIN337057)

Target: Isocitrate Dehydrogenase (IDH)

Reaktivität: Human, Schwein, Vicia faba

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Isocitrate Dehydrogenase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Isocitrate Dehydrogenase Antikörper

Spezifität: Binds both Dehydroepiandrosterone (DHEA) and DHEAS, demonstrating minimal cross-reactivity with Progesterone and Testosterone. DHEA is the most abundant hormone in the human body and a precursor of all the sex hormones. It is synthesized principally in the adrenal glands and gonads from cholesterol, through conversion to pregnenolone and 17a-Hydroxypregnenolone, by the action of P450 enzymes. DHEA is mainly found in serum as the sulphated form (DHEAS), a reversible reaction catalyzed by sulfotransferase in the adrenal glands, liver and small intestine.

Aufreinigung: Affinity purified

Immunogen: Purified isocitrate dehydrogenase from porcine heart.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:400 - 1:1600), IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Pig

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Store undiluted.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles. Store undiluted.

Details zu Isocitrate Dehydrogenase

Target: Isocitrate Dehydrogenase (IDH)