Prohibitin 2 Antikörper (AA 88-299)

Produktnummer ABIN337044

Der Kaninchen Polyklonal anti-Prohibitin 2 Antikörper (ABIN337044) detektiert spezifisch Prohibitin 2 in WB, IHC, IHC (p), IP und ICC. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Ratte.

Kurzübersicht für Prohibitin 2 Antikörper (AA 88-299) (ABIN337044)

Target: Prohibitin 2 (PHB2)

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Prohibitin 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-Prohibitin 2 Antikörper

Bindungsspezifität: AA 88-299

Spezifität: Recognizes mouse REA, Mr 37kD. Species cross-reactivity: human and rat.

Aufreinigung: Protein A purified

Immunogen: His6-tagged fusion protein corresponding to aa88-299 of human REA-1. The immunizing sequence is identical in mouse.

Type of Immunogen: Fusion protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IHC, IHC-P (5 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Immunocytochemistry. Proteins were visualized using a goat anti-rabbit IgG labeled with HRP and a chemiluminescence detection system. Immunoprecipitation: Immunoprecipitates REA from MCF-7 and 3T3 whole cell extracts. Immunocytochemistry: Nuclear staining of REA in paraformaldehyde fixed, Triton X-100 permeabilized MCF-7 cells.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-glycine, 0.15 M sodium chloride,  pH 7.4, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu Prohibitin 2

Target: Prohibitin 2 (PHB2)

Andere Bezeichnung: Prohibitin 2 / PHB2

Hintergrund: Name/Gene ID: PHB2
Family: Transcription factor

Synonyms: PHB2, BCAP37, BAP, D-prohibitin, p22, Prohibitin-2, B-cell associated protein, Bap37, PNAS-141, Prohibitin 2, REA

Gen-ID: 11331

UniProt: Q99623

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling