POLL Antikörper (AA 563-575)

Produktnummer ABIN337014

Der Ziege Polyklonal anti-POLL Antikörper (ABIN337014) detektiert spezifisch POLL in ELISA, IHC und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte, Maus, Kaninchen, Rind (Kuh), Schwein, Fledermaus, Hamster und Affe.

Kurzübersicht für POLL Antikörper (AA 563-575) (ABIN337014)

Target: POLL (Polymerase (DNA Directed), lambda (POLL))

Reaktivität: Human, Ratte, Maus, Kaninchen, Rind (Kuh), Schwein, Fledermaus, Hamster, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser POLL Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-POLL Antikörper

Bindungsspezifität: AA 563-575

Spezifität: Human POLL. It has been brought to our attention that this product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32 % in the core) but considering only the peptide that we used as immunogen the two share the sequence YREP**R** at the C-terminus. Further the protein structures appear to be very similar in this region.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Bovine, Bat, Hamster, Elephant, Panda, Rabbit, Pig (100%) Dog, Opossum, Turkey, Chicken (92%) Xenopus, Zebrafish, Sea anemone (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-LGLPYREPAERDW from the C-terminus of human POLL (NP_037406.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Bovine, Bat, Hamster, Elephant, Panda, Rabbit, Pig (100%), Dog, Opossum, Turkey, Chicken (92%), Xenopus, Zebrafish, Sea anemone (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu POLL

Target: POLL (Polymerase (DNA Directed), lambda (POLL))

Andere Bezeichnung: POLL / DNA Polymerase Lambda

Hintergrund: Name/Gene ID: POLL

Synonyms: POLL, DNA polymerase beta-2, DNA polymerase beta-N, DNA polymerase lambda, DNA polymerase kappa, Pol beta2, BETAN, Pol Lambda, POLKAPPA

Gen-ID: 27343

NCBI Accession: NP_037406

UniProt: Q9UGP5

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response